【資料下載】在線噴針清洗對持續(xù)Nano-ESI-LCMS的影響
Nanospray has become an essential tool in high-sensitivity MS, but limited robustness and reproducibility have historically challenged the adoption of nanospray in quantitative applications with triple quadrupole mass spectrometry. MS-based biomarker quantitation places strict requirements on the analytical performance of nanobore LC-MS not only in the need for reproducibility and robustness but also due to the intrinsically complex nature of the samples used in these workflows. Automated emitter positioning with rinsing has been previously demonstrated to improve spray stability and analyte response on a 3D ion trap MS1. Here we investigate the utility of automated tip rinsing to improve emitter spray stability and data quality on a hybrid triple quadrupole/LIT MS equipped with a heated interface blanketed by a laminar flow of nitrogen gas.
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